Liquid chromatography
Researcher: Maria-Loredana Soran
Keywords: Liquid chromatography, TLC, pollutants, organic compounds.
Description
Liquid chromatography is a laboratory technique that separates chemicals in a mixture, allowing their qualitative and quantitative determination. It is based on the different distribution of the components of a mixture between two phases: the stationary phase and the mobile phase. Liquid chromatography can be closed column or open column (Thin Layer Chromatography, TLC).
The most commonly used is closed column chromatography, in particular High Performance Liquid Chromatography (HPLC).
The main advantages of HPLC are:
- high separation capacity;
- high separation speed;
- possibility of continuous monitoring of the column eluent;
- repeated and reproducible analysis using the same stationary and mobile phases;
- automation of analytical procedures and data processing;
- non-destructive detection;
- after separation, substances can be collected in fraction collectors;
- easy scaling.
Applications
Areas of application: research and development, quality control (detection of impurities, stability under different environmental conditions, stability over time, etc.), analysis of environmental samples, chemical synthesis (separation of reaction products), determination of the composition of plant extracts, etc.
Systems: organic, inorganic and organometallic compounds.
Industries: pharmaceutical industry, chemical industry, food supplements industry, agri-food industry, cosmetics industry, environment, health.
Infrastructure
Our laboratory has a Shimadzu LCMS-2010 high-performance liquid chromatograph equipped with several detectors:
- diode-array detector (PDA),
- fluorescence detector (FD),
- refractive index detector (RID),
- mass spectrometer (MS),
to analyze a wide range of compounds.
We also have equipment for separations by thin-layer chromatography:
- Camag Linomat 5 semi-automatic applicator;
- Camag TLC Scanner 3 photodensitometer.
Advantages
INCDTIM offers R&D services based on high-performance liquid chromatography covering almost the full range of practical applications.
Prior to the conclusion of a contractual relationship, we offer consultancy to define as precisely as possible the needs of the client/partner.
On request, we offer extraction of the desired compound(s) from various matrices.
Existing facilities allow us to analyze a wide range of compounds.
Estimated costs
The total cost of CD services based on high-performance liquid chromatography depends on the type of analysis:
Qualitative analysis:
chromatograph running time, consumables and wear: 170 lei;
labor, which includes personnel and indirect costs associated with sample preparation operations, analysis and interpretation of results, preparation of analysis/research report: depending on the complexity of the study.
Quantitative analysis:
chromatograph running time, consumables and wear and tear: 170 lei;
calibration curve: 1000 lei;
labor, including personnel and indirect costs associated with sample preparation, analysis and interpretation of results, and preparation of analysis/research report: depending on the complexity of the study.